The cellular heat shock response monitored by chemical exchange saturation 
transfer MRI

Kleimaier, D; Goerke, S; Nies, C; Zaiss, M; Kunz, P; Gottwald, E; Schad, LR

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The cellular heat shock response monitored by chemical exchange saturation transfer MRI

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https://www.ismrm.org/20/program_files/DP08-01.htm
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Kleimaier, D., Goerke, S., Nies, C., Zaiss, M., Kunz, P., Gottwald, E., et al. (2020). The cellular heat shock response monitored by chemical exchange saturation transfer MRI. Poster presented at 2020 ISMRM & SMRT Virtual Conference & Exhibition.

Cite as: https://hdl.handle.net/21.11116/0000-0006-D8B8-7

Abstract

Chemical exchange saturation transfer of the relayed nuclear Overhauser effect(rNOE) enables detection of mobile protein protons via their exchange with the water signal. Recent studies have shown a close relationship between the rNOE-signal and the protein conformation. This study used the -3.5ppm rNOE-signal to monitor the heat shock response of HepG2 cells in a microcavity array-based bioreactor system. A significant drop of the rNOE-signal to 92.4±1.3% and a slow, steady increase of the rNOE-signal for 136.1±13.6min after heat shock were observed. Therefore, the rNOE-signal in CEST spectroscopic imaging is a sensitive readout for the cellular heat shock response.