Serial femtosecond and serial synchrotron crystallography yield data of 
equivalent quality: a systematic comparison

Mehrabi, P.; Bücker, R.; Bourenkov, G.; Ginn, H.; von Stetten, D.; Mueller-Werkmeister, H. M.; Kuo, A.; Morizumi, T.; Eger, B.; Ou, W.-L.; Oghbaey, S.; Sarracini, A.; Besaw, J.; Paré-Labrosse, O.; Meier, S.; Schikora, H.; Tellkamp, F.; Marx, A.; Sherrell, D. A.; Axford, D.; Owen, R. L.; Ernst, O. P.; Pai, Emil F.; Schulz, E.-C.; Miller, R. J. D.

doi:10.1101/2020.08.21.257170

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

このアイテムの新しいバージョンが利用可能です:
https://pure.mpg.de/pubman/item/item_3249417_6

詳細要約

公開

成果報告書

Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison

MPS-Authors

/persons/resource/persons209117

Mehrabi, P.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Department of Medical Biophysics, University of Toronto;
The Campbell Family Cancer Research Institute, Ontario, Cancer Institute;
Center for Free-Electron Laser Science;

/persons/resource/persons136038

Bücker, R.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons196452

Paré-Labrosse, O.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Departments of Chemistry and Physics, University of Toronto;

/persons/resource/persons241303

Schikora, H.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons182596

Tellkamp, F.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons136037

Marx, A.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons202768

Schulz, E.-C.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science;

/persons/resource/persons136024

Miller, R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Departments of Chemistry and Physics, University of Toronto;
Department of Physics, Universität Hamburg;
Center for Free-Electron Laser Science;

External Resource

https://dx.doi.org/10.1101/2020.08.21.257170
(プレプリント)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

2020.08.21.257170v1.full.pdf
(プレプリント), 4MB

付随資料 (公開)

There is no public supplementary material available

引用

Mehrabi, P., Bücker, R., Bourenkov, G., Ginn, H., von Stetten, D., Mueller-Werkmeister, H. M., Kuo, A., Morizumi, T., Eger, B., Ou, W.-L., Oghbaey, S., Sarracini, A., Besaw, J., Paré-Labrosse, O., Meier, S., Schikora, H., Tellkamp, F., Marx, A., Sherrell, D. A., Axford, D., Owen, R. L., Ernst, O. P., Pai, E. F., Schulz, E.-C., & Miller, R. J. D. (2020). Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison. doi:10.1101/2020.08.21.257170.

引用: https://hdl.handle.net/21.11116/0000-0006-E333-0

要旨

For the two proteins myoglobin (MB) and fluoroacetate dehalogenase (FAcD), we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of crystals, the same sample delivery device, as well as the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins reasonable data statistics can be obtained with approximately 5000 room temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that diffraction quality is rather linked to the properties of the crystals than to the radiation source. Time-resolved experiments can therefore be conducted at the source that best matches the desired time-resolution.