Compaction of RNA duplexes in the cell

Collauto, Alberto; von Bülow, Sören; Gophane, Dnyaneshwar; Saha, Subham; Stelzl, Lukas S.; Hummer, Gerhard; Sigurdsson, Snorri Th.; Prisner, Thomas

doi:10.1002/anie.202009800

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Compaction of RNA duplexes in the cell

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von Bülow, Sören
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;

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Stelzl, Lukas S.
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;

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Hummer, Gerhard
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;
Institute for Biophysics, Goethe University Frankfurt, Frankfurt am Main, Germany;

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引用

Collauto, A., von Bülow, S., Gophane, D., Saha, S., Stelzl, L. S., Hummer, G., Sigurdsson, S. T., & Prisner, T. (2020). Compaction of RNA duplexes in the cell. Angewandte Chemie, International Edition in English, 59, 23025-23029. doi:10.1002/anie.202009800.

引用: https://hdl.handle.net/21.11116/0000-0006-E87E-8

要旨

The structure and flexibility of RNA depend sensitively on the microenvironment. Using pulsed electron-electron double-resonance (PELDOR) spectroscopy combined with advanced labeling techniques, we show that the structure of double-stranded RNA (dsRNA) changes upon internalization into Xenopus laevis oocytes. Compared to dilute solution, the dsRNA A-helix is more compact in cells. We recapitulate this compaction in a densely crowded protein solution. Atomic-resolution molecular dynamics simulations of dsRNA capture semi-quantitatively the compaction, and identify non-specific electrostatic interactions between proteins and dsRNA as a possible driver of this effect.