Comprehensive genome data analysis establishes a triple whammy of 
carbapenemases, ICEs and multiple clinically relevant bacteria

Botelho, João; Mourão, Joana; Roberts, Adam P; Peixe, Luísa

doi:10.1099/mgen.0.000424

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_3250813_2

DetailsSummary

Released

Journal Article

Comprehensive genome data analysis establishes a triple whammy of carbapenemases, ICEs and multiple clinically relevant bacteria

MPS-Authors

/persons/resource/persons241500

Botelho, João
Max Planck Fellow Group Antibiotic Resistance Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

mgen000424.pdf
(Publisher version), 2MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Botelho, J., Mourão, J., Roberts, A. P., & Peixe, L. (2020). Comprehensive genome data analysis establishes a triple whammy of carbapenemases, ICEs and multiple clinically relevant bacteria. Microbial Genomics, 000424, pp. 1-10. doi:10.1099/mgen.0.000424.

Cite as: https://hdl.handle.net/21.11116/0000-0006-F1AF-5

Abstract

Carbapenemases inactivate most β-lactam antibiotics, including carbapenems, and have frequently been reported among
Enterobacteriaceae, Acinetobacter spp. and Pseudomonas spp. Traditionally, the horizontal gene transfer of carbapenemaseencoding genes (CEGs) has been linked to plasmids. However, given that integrative and conjugative elements (ICEs) are possibly the most abundant conjugative elements among prokaryotes, we conducted an in silico analysis to ascertain the likely role
of ICEs in the spread of CEGs among all bacterial genomes (n=182663). We detected 17 520 CEGs, of which 66 were located
within putative ICEs among several bacterial species (including clinically relevant bacteria, such as Pseudomonas aeruginosa,
Klebsiella pneumoniae and Escherichia coli). Most CEGs detected within ICEs belong to the IMP, NDM and SPM metallo-betalactamase families, and the serine beta-lactamase KPC and GES families. Different mechanisms were likely responsible for
acquisition of these genes. The majority of CEG-bearing ICEs belong to the MPFG, MPFT
and MPFF
classes and often encode
resistance to other antibiotics (e.g. aminoglycosides and fluoroquinolones). This study provides a snapshot of the different CEGs
associated with ICEs among available bacterial genomes and sheds light on the underappreciated contribution of ICEs to the
spread of carbapenem resistance globally.