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The biophysical, molecular, and anatomical landscape of pigeon CRY4: A candidate light-based quantal magnetosensor

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Malkemper,  E. Pascal
Max Planck Research Group Neurobiology of Magnetoreception, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Citation

Hochstoeger, T., Al Said, T., Maestre, D., Walter, F., Vilceanu, A., Pedron, M., et al. (2020). The biophysical, molecular, and anatomical landscape of pigeon CRY4: A candidate light-based quantal magnetosensor. Science Advances, 6(33): eabb9110. doi:10.1126/sciadv.abb9110.


Cite as: http://hdl.handle.net/21.11116/0000-0006-F847-3
Abstract
The biophysical and molecular mechanisms that enable animals to detect magnetic fields are unknown. It has been proposed that birds have a light-dependent magnetic compass that relies on the formation of radical pairs within cryptochrome molecules. Using spectroscopic methods, we show that pigeon cryptochrome clCRY4 is photoreduced efficiently and forms long-lived spin-correlated radical pairs via a tetrad of tryptophan residues. We report that clCRY4 is broadly and stably expressed within the retina but enriched at synapses in the outer plexiform layer in a repetitive manner. A proteomic survey for retinal-specific clCRY4 interactors identified molecules that are involved in receptor signaling, including glutamate receptor-interacting protein 2, which colocalizes with clCRY4. Our data support a model whereby clCRY4 acts as an ultraviolet-blue photoreceptor and/or a light-dependent magnetosensor by modulating glutamatergic synapses between horizontal cells and cones.