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Identification of cross-linked peptides from large sequence databases

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Citation

Rinner, O., Seebacher, J., Walzthoeni, T., Mueller, L. N., Beck, M., Schmidt, A., et al. (2008). Identification of cross-linked peptides from large sequence databases. Nature Methods, 5(4), 315-318. doi:10.1038/nmeth.1192.


Cite as: http://hdl.handle.net/21.11116/0000-0007-0264-6
Abstract
We describe a method to identify cross-linked peptides from complex samples and large protein sequence databases by combining isotopically tagged cross-linkers, chromatographic enrichment, targeted proteomics and a new search engine called xQuest. This software reduces the search space by an upstream candidate-peptide search before the recombination step. We showed that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search.