The symbiotically essential cbb3-type oxidase of Bradyrhizobium japonicum is a 
proton pump

Arslan, Engin; Kannt, Aimo; Thöny-Meyer, Linda; Hennecke, Hauke

doi:10.1016/S0014-5793(00)01277-1

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

The symbiotically essential cbb₃-type oxidase of Bradyrhizobium japonicum is a proton pump

MPS-Authors

/persons/resource/persons137733

Kannt, Aimo
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Arslan, E., Kannt, A., Thöny-Meyer, L., & Hennecke, H. (2000). The symbiotically essential cbb₃-type oxidase of Bradyrhizobium japonicum is a proton pump. FEBS Letters, 470(1), 7-10. doi:10.1016/S0014-5793(00)01277-1.

Cite as: https://hdl.handle.net/21.11116/0000-0007-027B-D

Abstract

Purified cbb₃-type oxidase of Bradyrhizobium japonicum was reconstituted into phospholipid vesicles. Tight vesicles were obtained as shown by the disturbance of ΔpH with CCCP and the membrane potential with valinomycin, which led to a six-fold increase in cytochrome c oxidase activity. The vesicles were thus suitable for proton translocation experiments. In the presence of valinomycin, a pulse with reduced cytochrome c caused an acidification with a subsequent alkalinization, whereas the same pulse caused only an alkalinization in the presence of valinomycin plus CCCP. We conclude that the cbb₃-type oxidase of B. japonicum is a proton pump.