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Journal Article

Luminal particles within cellular microtubules

MPS-Authors

Garvalov,  Boyan K.
Max Planck Research Group: Axonal Growth and Regeneration / Bradke, MPI of Neurobiology, Max Planck Society;

Gruska,  Manuela
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Beck,  Martin
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Leis,  Andrew
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Bradke,  Frank
Max Planck Research Group: Axonal Growth and Regeneration / Bradke, MPI of Neurobiology, Max Planck Society;

Baumeister,  Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Cyrklaff,  Marek
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Garvalov, B. K., Zuber, B., Bouchet-Marquis, C., Kudryashev, M., Gruska, M., Beck, M., et al. (2006). Luminal particles within cellular microtubules. The Journal of Cell Biology, 174(6), 759-765. doi:10.1083/jcb.200606074.


Cite as: https://hdl.handle.net/21.11116/0000-0007-0498-9
Abstract
The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate with the internal side of microtubules. We used cryoelectron tomography to investigate in a quantitative manner the three dimensional structure of microtubules in intact mammalian cells. We show that the lumen of microtubules in this native state is filled with discrete, globular particles with a diameter of 7 nm and spacings between 8 and 20 nm in neuronal cells. Cross-sectional views of microtubules confirm the presence of luminal material in vitreous sections of brain tissue. Most of the luminal particles had connections to the microtubule wall, as revealed in tomograms. A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells.