English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography

MPS-Authors
/persons/resource/persons232071

Beck,  Martin       
Max Planck Institute of Biochemistry, Max Planck Society;

Förster,  Friedrich
Max Planck Institute of Biochemistry, Max Planck Society;

Ecke,  Mary
Max Planck Institute of Biochemistry, Max Planck Society;

Plitzko,  Jürgen M.
Max Planck Institute of Biochemistry, Max Planck Society;

Melchior,  Frauke
Max Planck Institute of Biochemistry, Max Planck Society;

Gerisch,  Günther
Max Planck Institute of Biochemistry, Max Planck Society;

Baumeister,  Wolfgang
Max Planck Institute of Biochemistry, Max Planck Society;

Medalia,  Ohad
Max Planck Institute of Biochemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Beck, M., Förster, F., Ecke, M., Plitzko, J. M., Melchior, F., Gerisch, G., et al. (2004). Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Science, 306(5700), 1387-1390. doi:10.1126/science.1104808.


Cite as: https://hdl.handle.net/21.11116/0000-0007-0540-B
Abstract
Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.