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The light-harvesting complex II (B800/850) from Rhodospirillum molischianum is an octamer

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Germeroth,  Lothar
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Michel,  Hartmut       
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Kleinekofort, W., Germeroth, L., van den Broek, J. A., Schubert, D., & Michel, H. (1992). The light-harvesting complex II (B800/850) from Rhodospirillum molischianum is an octamer. Biochimica et Biophysica Acta, Bioenergetics, 1140(1), 102-104. doi:10.1016/0005-2728(92)90025-W.


Cite as: https://hdl.handle.net/21.11116/0000-0007-05C7-3
Abstract
The molar mass of the light-harvesting complex II (LH II) from Rhodospirillum molischianum in solutions of two nonionic detergents (N,N-dimethyldodecylamine N-oxide, nonaethyleneglycol lauryl ether) was studied by sedimentation equilibrium experiments in the analytical ultracentrifuge. To account for the contribution of protein-bound detergent to the effective particle mass, solvent density was varied by addition of D<sub>2<(sub>O. In both detergents, the relative molar mass of the protein/pigment complex was found to be 114 500 ± 7% (maximum error), corresponding to the (8.0 ± 0.6)-fold of that of the monomeric unit. The complex is thus the octamer of the basic unit.