English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Fortified coiled coils : enhancing mechanical stability with lactam or metal staples

MPS-Authors
/persons/resource/persons228508

Tunn,  Isabell
Kerstin Blank, Mechano(bio)chemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons134416

Blank,  Kerstin G.
Kerstin Blank, Mechano(bio)chemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

External Ressource

primary data
(Supplementary material)

Fulltext (public)

Article.pdf
(Publisher version), 2MB

Article_de.pdf
(Publisher version), 2MB

Supplementary Material (public)
There is no public supplementary material available
Citation

López-García, P., de Araujo, A. D., Bergues-Pupo, A. E., Tunn, I., Fairlie, D. P., & Blank, K. G. (2021). Fortified coiled coils: enhancing mechanical stability with lactam or metal staples. Angewandte Chemie International Edition, 60(1), 232-236. doi:10.1002/anie.202006971.


Cite as: http://hdl.handle.net/21.11116/0000-0007-0CA0-7
Abstract
Coiled coils (CCs) are powerful supramolecular building blocks for biomimetic materials, increasingly used for their mechanical properties. Here, we introduce helix-inducing macrocyclic constraints, so-called staples, to tune thermodynamic and mechanical stability of CCs. We show that thermodynamic stabilization of CCs against helix uncoiling primarily depends on the number of staples, whereas staple positioning controls CC mechanical stability. Inserting a covalent lactam staple at one key force application point significantly increases the barrier to force-induced CC dissociation and reduces structural deformity. A reversible His-Ni 2+ -His metal staple also increases CC stability, but ruptures upon mechanical loading to allow helix uncoiling. Staple type, position and number are key design parameters in using helical macrocyclic templates for fine-tuning CC properties in emerging biomaterials.