Determination of the molar mass of pigment-containing complexes of intrinsic 
membrane proteins: Problems, solutions and application to the light-harvesting 
complex B800/820 of Rhodospirillum molischianum

Schubert , D.; Tziatzios, C.; van den Broek, J.A.; Schuck, P.; Germeroth, Lothar; Michel, Hartmut

doi:10.1007/BFb0115598

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Determination of the molar mass of pigment-containing complexes of intrinsic membrane proteins: Problems, solutions and application to the light-harvesting complex B800/820 of Rhodospirillum molischianum

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Schubert , D.
Institut für Biophysik, Johann-Wolfgang Goethe-Universität Frankfurt, Frankfurt am Main, Germany;
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Germeroth, Lothar
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Michel, Hartmut
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Schubert, D., Tziatzios, C., van den Broek, J., Schuck, P., Germeroth, L., & Michel, H. (1993). Determination of the molar mass of pigment-containing complexes of intrinsic membrane proteins: Problems, solutions and application to the light-harvesting complex B800/820 of Rhodospirillum molischianum. Dr. Dietrich Steinkopff Verlag GmbH & Co. KG. doi:10.1007/BFb0115598.

Cite as: https://hdl.handle.net/21.11116/0000-0007-0A37-1

Abstract

An adaptation of a procedure for the simultaneous determination of partial specific volumes and molar masses by sedimentation equilibrium experiments (Edelstein SJ, Schachman HK (1967) J Biol Chem 242: 306–311) to intrinsic membrane proteins in detergent solutions is described. The method requires (1) that the protein is available in at least two detergents the densities of which differ as much as possible, and (2) that the effective molar masses of the mixed protein/detergent micelles, M(1 − vρ 0), are determined under conditions of density matching. The method is applied to a pigment-containing membrane protein, the light-harvesting complex B800/820 (LH III) from Rhodospirillum molischianum, in solutions of three detergents. It is shown that the complex is the hexamer of the basic α/β/pigment unit.