Three molecules of ubiquinone bind specifically to mitochondrial cytochrome bc1 
complex

Bartoschek, Stefan; Johansson, Malin; Geierstanger, Bernhard H.; Okun, jürgen G.; Lancaster, C. Roy D.; Humpfer, Eberhard; Yu, Linda; Yu, Chang-An; Griesinger, Christian; Brandt, Ulrich

doi:10.1074/jbc.c100365200

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Three molecules of ubiquinone bind specifically to mitochondrial cytochrome bc₁ complex

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Lancaster, C. Roy D.
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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引用

Bartoschek, S., Johansson, M., Geierstanger, B. H., Okun, j. G., Lancaster, C. R. D., Humpfer, E., Yu, L., Yu, C.-A., Griesinger, C., & Brandt, U. (2001). Three molecules of ubiquinone bind specifically to mitochondrial cytochrome bc₁ complex. The Journal of Biological Chemistry, 276(38), 35231-35234. doi:10.1074/jbc.c100365200.

引用: https://hdl.handle.net/21.11116/0000-0007-0AEF-2

要旨

Bifurcated electron flow to high potential "Rieske" iron-sulfur cluster and low potential heme b_L is crucial for respiratory energy conservation by the cytochrome bc₁ complex. The chemistry of ubiquinol oxidation has to ensure the thermodynamically unfavorable electron transfer to heme b_L. To resolve a central controversy about the number of ubiquinol molecules involved in this reaction, we used high resolution magic-angle-spinning nuclear magnetic resonance experiments to show that two out of three n-decyl-ubiquinones bind at the ubiquinol oxidation center of the complex. This substantiates a proposed mechanism in which a charge transfer between a ubiquinol/ubiquinone pair explains the bifurcation of electron flow.