Visualization of Multimerization and Self-Assembly of DNA-Functionalized Gold 
Nanoparticles Using In-Liquid Transmission Electron Microscopy

Keskin, S.; Besztejan, Stephanie; Kassier, G.; Manz, S.; Bücker, R.; Riekeberg, S.; Trieu, H. K.; Rentmeister, A.; Miller, R. J. D.

doi:10.1021/acs.jpclett.5b02075

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Visualization of Multimerization and Self-Assembly of DNA-Functionalized Gold Nanoparticles Using In-Liquid Transmission Electron Microscopy

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Keskin, S.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Besztejan, Stephanie
University of Hamburg, Chemistry Department, Institute for Biochemistry and Molecular Biology, Martin-Luther-King Platz 6, 20146 Hamburg, Germany;
The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg, Germany;
International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Kassier, G.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Manz, S.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Bücker, R.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Miller, R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg, Germany;
Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada;

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http://dx.doi.org/10.1021/acs.jpclett.5b02075
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Citation

Keskin, S., Besztejan, S., Kassier, G., Manz, S., Bücker, R., Riekeberg, S., et al. (2015). Visualization of Multimerization and Self-Assembly of DNA-Functionalized Gold Nanoparticles Using In-Liquid Transmission Electron Microscopy. The Journal of Physical Chemistry Letters, 6(22), 4487-4492. doi:10.1021/acs.jpclett.5b02075.

Cite as: https://hdl.handle.net/21.11116/0000-0007-1030-0

Abstract

Base-pairing stability in DNA-gold nanoparticle (DNA-AuNP) multimers along with their dynamics under different electron beam intensities was investigated with in-liquid transmission electron microscopy (in-liquid TEM). Multimer formation was triggered by hybridization of DNA oligonucleotides to another DNA strand (Hyb-DNA) related to the concept of DNA origami. We analyzed the degree of multimer formation for a number of samples and a series of control samples to determine the specificity of the multimerization during the TEM imaging. DNA-AuNPs with Hyb-DNA showed an interactive motion and assembly into 1D structures once the electron beam intensity exceeds a threshold value. This behavior was in contrast with control studies with noncomplementary DNA linkers where statistically significantly reduced multimerization was observed and for suspensions of citrate-stabilized AuNPs without DNA, where we did not observe any significant motion or aggregation. These findings indicate that DNA base-pairing interactions are the driving force for multimerization and suggest a high stability of the DNA base pairing even under electron exposure.