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Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy

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Gorbulev,  Valentin
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Akhundova,  Aida
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Büchner,  Hubert
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Fahrenholz,  Falk
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Gorbulev, V., Akhundova, A., Büchner, H., & Fahrenholz, F. (1992). Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy. European Journal of Biochemistry, 208(2), 405-410. doi:10.1111/j.1432-1033.1992.tb17201.x.


Cite as: http://hdl.handle.net/21.11116/0000-0007-9922-6
Abstract
The homology screening approach has been used to clone a new member of the guanine-nucleotide-binding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52% and 47% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Binding experiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confirmed the bombesin-like nature of the cloned receptor. The relative order of ligand affinity, GRP = neuromedin C much greater than neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.