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Journal Article

FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps

MPS-Authors
/persons/resource/persons192369

Ganzinger,  Kristina A.
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons240609

Merino-Salomon,  Adrian
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons215956

Garcia-Soriano,  Daniela A.
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons243677

Butterfield,  Nelson A.
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons137778

Litschel,  Thomas
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78705

Siedler,  Frank
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons15815

Schwille,  Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

https://doi.org/10.1101/791459
(Preprint)

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Fulltext (public)

anie.202001928.pdf
(Publisher version), 2MB

Supplementary Material (public)

anie202001928-sup-0001-misc_information.pdf
(Supplementary material), 4MB

Citation

Ganzinger, K. A., Merino-Salomon, A., Garcia-Soriano, D. A., Butterfield, N. A., Litschel, T., Siedler, F., et al. (2020). FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps. Angewandte Chemie International Edition, 59(48), 21372-21376. doi:10.1002/anie.202001928.


Cite as: https://hdl.handle.net/21.11116/0000-0008-47AE-5
Abstract
The geometry of reaction compartments can affect the local outcome of interface-restricted reactions. Giant unilamellar vesicles (GUVs) are commonly used to generate cell-sized, membrane-bound reaction compartments, which are, however, always spherical. Herein, we report the development of a microfluidic chip to trap and reversibly deform GUVs into cigar-like shapes. When trapping and elongating GUVs that contain the primary protein of the bacterial Z ring, FtsZ, we find that membrane-bound FtsZ filaments align preferentially with the short GUV axis. When GUVs are released from this confinement and membrane tension is relaxed, FtsZ reorganizes reversibly from filaments into dynamic rings that stabilize membrane protrusions; a process that allows reversible GUV deformation. We conclude that microfluidic traps are useful for manipulating both geometry and tension of GUVs, and for investigating how both affect the outcome of spatially-sensitive reactions inside them, such as that of protein self-organization.