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Journal Article

Super-resolution spatial proximity detection with proximity-PAINT.

MPS-Authors
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Schueder,  Florian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Lara-Gutiérrez,  Juanita
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Haas,  Daniel
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Jungmann,  Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

Fulltext (public)

anie.202009031.pdf
(Publisher version), 2MB

Supplementary Material (public)
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Citation

Schueder, F., Lara-Gutiérrez, J., Haas, D., Beckwith, K. S., Yin, P., Ellenberg, J., et al. (2020). Super-resolution spatial proximity detection with proximity-PAINT. Angewandte Chemie, International Edition in English. doi:10.1002/anie.202009031.


Cite as: http://hdl.handle.net/21.11116/0000-0007-7E44-0
Abstract
Visualizing the functional interactions of biomolecules such as proteins and nucleic acids is key to understanding cellular life on the molecular scale. Spatial proximity is often used as a proxy for the direct interaction of biomolecules. However, current techniques to visualize spatial proximity are either limited by spatial resolution, dynamic range, or lack of single-molecule sensitivity. Here, we introduce Proximity-PAINT (pPAINT), a variation of the super-resolution microscopy technique DNA-PAINT. pPAINT uses a split-docking-site configuration to detect spatial proximity with high sensitivity, low false-positive rates, and tunable detection distances. We benchmark and optimize pPAINT using designer DNA nanostructures and demonstrate its cellular applicability by visualizing the spatial proximity of alpha- and beta-tubulin in microtubules using super-resolution detection. © 2020 Wiley-VCH GmbH.