Inhibition and labeling of the rat renal Na+/H+-exchanger by an antagonist of 
muscarinic acetylcholine receptors

Friedrich, Thomas; Burckhardt, Gerhard

doi:10.1016/S0006-291X(88)80962-8

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Inhibition and labeling of the rat renal Na⁺/H⁺-exchanger by an antagonist of muscarinic acetylcholine receptors

MPS-Authors

/persons/resource/persons251310

Burckhardt, Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Friedrich, T., & Burckhardt, G. (1988). Inhibition and labeling of the rat renal Na⁺/H⁺-exchanger by an antagonist of muscarinic acetylcholine receptors. Biochemical and Biophysical Research Communications, 157(3), 921-929. doi:10.1016/S0006-291X(88)80962-8.

Cite as: https://hdl.handle.net/21.11116/0000-0007-4B4A-3

Abstract

A covalently binding label for muscarinic acetylcholine receptors, propylbenzilylcholine mustard (PrBCM), irreversibly inhibits the Na⁺/H⁺ exchanger in rat renal brush-border membrane vesicles. Substrates of the antiporter, Na⁺ and Li⁺, as well as inhibitors, amiloride, 5-(N-ethyl-N-isopropyl)amiloride (EIPA) and propranolol, protect the antiporter from inactivation by PrBCM. With [³H]PrBCM a band with an app. M_r of 65 kDa is predominantly labeled. Amiloride protects this band from labeling with [³H]PrBCM and [¹⁴C]-N,N′-dicyclohexylcarbodiimide (DCCD) proving its identity with the renal Na⁺/H⁺ exchanger. Our data reveal a specific interaction of PrBCM with the Na⁺/H⁺ exchanger and suggest structural relations between antiporter and receptors.