Hybrid structural model of the complete human ESCRT-0 complex

Ren, Xuefeng; Kloer, Daniel P.; Kim, Young C.; Ghirlando, Rodolfo; Saidi, Layla F.; Hummer, Gerhard; Hurley, James H.

doi:10.1016/j.str.2009.01.012

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Hybrid structural model of the complete human ESCRT-0 complex

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Citation

Ren, X., Kloer, D. P., Kim, Y. C., Ghirlando, R., Saidi, L. F., Hummer, G., et al. (2009). Hybrid structural model of the complete human ESCRT-0 complex. Structure, 17(3), 406-416. doi:10.1016/j.str.2009.01.012.

Cite as: https://hdl.handle.net/21.11116/0000-0007-4B66-3

Abstract

The human Hrs and STAM proteins comprise the ESCRT-0 complex, which sorts ubiquitinated cell surface receptors to lysosomes for degradation. Here we report a model for the complete ESCRT-0 complex based on the crystal structure of the Hrs-STAM core complex, previously solved domain structures, hydrodynamic measurements, and Monte Carlo simulations. ESCRT-0 expressed in insect cells has a hydrodynamic radius of RH = 7.9 nm and is a 1:1 heterodimer. The 2.3 Angstroms crystal structure of the ESCRT-0 core complex reveals two domain-swapped GAT domains and an antiparallel two-stranded coiled-coil, similar to yeast ESCRT-0. ESCRT-0 typifies a class of biomolecular assemblies that combine structured and unstructured elements, and have dynamic and open conformations to ensure versatility in target recognition. Coarse-grained Monte Carlo simulations constrained by experimental RH values for ESCRT-0 reveal a dynamic ensemble of conformations well suited for diverse functions.