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SEF1 and VMA1 Genes Regulate Riboflavin Biosynthesis in the Flavinogenic Yeast Candida Famata

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Lyzak,  O.
Zachariae, Wolfgang / Chromosome Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Andreieva, Y., Lyzak, O., Liu, W., Kang, Y., Dmytruk, K., & Sibirny, A. (2020). SEF1 and VMA1 Genes Regulate Riboflavin Biosynthesis in the Flavinogenic Yeast Candida Famata. Cytology and Genetics, 54(5), 379-385. doi:10.3103/S0095452720050023.


Cite as: http://hdl.handle.net/21.11116/0000-0007-4CD3-6
Abstract
Riboflavin (vitamin B-2) is an important component of the diet of living organisms since it is a precursor of flavin coenzymes FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide) involved in numerous enzymatic reactions. It is known that flavinogenic yeastC. famatais able to perform riboflavin overproduction under conditions of iron deficiency, but the regulation of this process remains unknown. It was shown that the deletion of the SEF1 gene (encoding transcription activator) blocked the ability for riboflavin overproduction under conditions of iron deficiency. It was determined that SEF1 promoters of other flavinogenic yeasts (Candida albicansandCandida tropicalis) fused with SEF1 ORF ofC. famatacan restore the overproduction of riboflavin in the sef1 Delta mutant. The disruption of the VMA1 gene (encoding the vacuolar ATPase subunit A) led to overproduction of riboflavin in C. famatain iron complete medium.