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Comparative genome analysis of 33 chlamydia strains reveals characteristic features of Chlamydia Psittaci and closely related species

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Barf,  Lisa-Marie
Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society;

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Hoelzer_Comparative_Pathogens_2020.pdf
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Hoelzer_Comparative_Pathogens_2020_Suppl.zip
(Supplementary material), 2MB

Citation

Hölzer, M., Barf, L.-M., Lamkiewicz, K., Vorimore, F., Lataretu, M., Favaroni, A., et al. (2020). Comparative genome analysis of 33 chlamydia strains reveals characteristic features of Chlamydia Psittaci and closely related species. Pathogens, 9(11): 899. doi:10.3390/pathogens9110899.


Cite as: http://hdl.handle.net/21.11116/0000-0007-6157-A
Abstract
To identify genome-based features characteristic of the avian and human pathogen Chlamydia (C.) psittaci and related chlamydiae, we analyzed whole-genome sequences of 33 strains belonging to 12 species. Using a novel genome analysis tool termed Roary ILP Bacterial Annotation Pipeline (RIBAP), this panel of strains was shown to share a large core genome comprising 784 genes and representing approximately 80 of individual genomes. Analyzing the most variable genomic sites, we identified a set of features of C. psittaci that in its entirety is characteristic of this species: (i) a relatively short plasticity zone of less than 30,000 nt without a tryptophan operon (also in C. abortus, C. avium, C. gallinacea, C. pneumoniae), (ii) a characteristic set of of Inc proteins comprising IncA, B, C, V, X, Y (with homologs in C. abortus, C. caviae and C. felis as closest relatives), (iii) a 502-aa SinC protein, the largest among Chlamydia spp., and (iv) an elevated number of Pmp proteins of subtype G (14 in C. psittaci, 14 in Cand. C. ibidis). In combination with future functional studies, the common and distinctive criteria revealed in this study provide important clues for understanding the complexity of host-specific behavior of individual Chlamydia spp.