Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ

Raddaoui, Nada; Stazzoni, Samuele; Möckl, Leonhard; Viverge, Bastien; Geiger, Florian; Engelke, Hanna; Braeuchle, Christoph; Carell, Thomas

doi:10.1002/cbic.201700209

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Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ

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Raddaoui, N., Stazzoni, S., Möckl, L., Viverge, B., Geiger, F., Engelke, H., et al. (2017). Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ. ChemBioChem, 18(17), 1716-1720. doi:10.1002/cbic.201700209.

Cite as: http://hdl.handle.net/21.11116/0000-0007-6836-8

Abstract

The in vivo incorporation of alkyne-modified bases into the genome of cells is today the basis for the efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilised. The incorporated alkynes are then efficiently detected by using azide-containing fluorophores and the Cu-I-catalysed alkyne-azide click reaction. For a world in which constant improvement in the sensitivity of a given method is driving diagnostic advancement, we developed azide- and alkyne-modified dendrimers that allow the establishment of sandwich-type detection assays that show significantly improved signal intensities and signal-to-noise ratios far beyond that which is currently possible.