English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ

MPS-Authors
There are no MPG-Authors available
External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Raddaoui, N., Stazzoni, S., Möckl, L., Viverge, B., Geiger, F., Engelke, H., et al. (2017). Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ. ChemBioChem, 18(17), 1716-1720. doi:10.1002/cbic.201700209.


Cite as: http://hdl.handle.net/21.11116/0000-0007-6836-8
Abstract
The in vivo incorporation of alkyne-modified bases into the genome of cells is today the basis for the efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilised. The incorporated alkynes are then efficiently detected by using azide-containing fluorophores and the Cu-I-catalysed alkyne-azide click reaction. For a world in which constant improvement in the sensitivity of a given method is driving diagnostic advancement, we developed azide- and alkyne-modified dendrimers that allow the establishment of sandwich-type detection assays that show significantly improved signal intensities and signal-to-noise ratios far beyond that which is currently possible.