The glycocalyx regulates the uptake of nanoparticles by human endothelial cells 
in vitro

Möckl, Leonhard; Hirn, Stephanie; Torrano, Adriano A.; Uhl, Bernd; Braeuchle, Christoph; Krombach, Fritz

doi:10.2217/nnm-2016-0332

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

The glycocalyx regulates the uptake of nanoparticles by human endothelial cells in vitro

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Möckl, L., Hirn, S., Torrano, A. A., Uhl, B., Braeuchle, C., & Krombach, F. (2017). The glycocalyx regulates the uptake of nanoparticles by human endothelial cells in vitro. Nanomedicine, 12(3), 207-217. doi:10.2217/nnm-2016-0332.

Cite as: https://hdl.handle.net/21.11116/0000-0007-69BF-D

Abstract

Aim: To assess the role of the endothelial glycocalyx (eGCX) for the uptake of nanoparticles by endothelial cells. Methods: The expression of the eGCX on cultured human umbilical vein endothelial cells was determined by immunostaining of heparan sulfate. Enzymatic degradation of the eGCX was achieved by incubating the cells with eGCX-shedding enzymes. The uptake of 50-nm polystyrene nanospheres was quantified by confocal microscopy. Results: Human umbilical vein endothelial cells expressed a robust eGCX when cultured for 10 days. The uptake of both carboxylated and aminated polystyrene nanospheres was significantly increased in cells in which the glycocalyx was enzymatically degraded, while it remained at a low level in cells with an intact glycocalyx. Conclusion: The eGCX constitutes a barrier against the internalization of blood-borne nanoparticles by endothelial cells.