En route from artificial to natural: Evaluation of inhibitors of 
mannose-specific adhesion of E. coli under flow

Möckl, Leonhard; Fessele, Claudia; Despras, Guillaume; Braeuchle, Christoph; Lindhorst, Thisbe K.

doi:10.1016/j.bbagen.2016.06.021

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsStatisticsRelease HistoryDetailsSummary

Released

Journal Article

En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow

MPS-Authors

There are no MPG-Authors available

External Ressource

No external resources are shared

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Möckl, L., Fessele, C., Despras, G., Braeuchle, C., & Lindhorst, T. K. (2016). En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow. Biochimica et Biophysica Acta-General Subjects, 1860(9), 2031-2036. doi:10.1016/j.bbagen.2016.06.021.

Cite as: http://hdl.handle.net/21.11116/0000-0007-6839-5

Abstract

We investigated the properties of six Escherichia coli adhesion inhibitors under static and under flow conditions. On mannan-covered model substrates and under static conditions, all inhibitors were able to almost completely abolish lectin-mediated E. coli adhesion. On a monolayer of living human microvascular endothelial cells (HMEC-1), the inhibitors reduced adhesion under static conditions as well, but a large fraction of bacteria still managed to adhere even at highest inhibitor concentrations. In contrast, under flow conditions E. coli did not exhibit any adhesion to HMEC-1 not even at inhibitor concentrations where significant adhesion was detected under static conditions. This indicates that the presence of shear stress strongly affects inhibitor properties and must be taken into account when evaluating the potency of bacterial adhesion inhibitors. (C) 2016 Elsevier B.V. All rights reserved.