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The Transport Activity of the Light Driven Ion Pump Halorhodopsin is regulated by Green and Blue Light

MPS-Authors

Hegemann,  Peter
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Oesterhelt,  Dieter
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Bamberg,  Ernst
Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Hegemann, P., Oesterhelt, D., & Bamberg, E. (1985). The Transport Activity of the Light Driven Ion Pump Halorhodopsin is regulated by Green and Blue Light. Biochimica et Biophysica Acta-Biomembranes, 819(2), 195-205. doi:10.1016/0005-2736(85)90174-9.


Cite as: http://hdl.handle.net/21.11116/0000-0007-A683-9
Abstract
Halorhodopsin-containing membrane fragments or purified halorhodopsin-containing asolectin vesicles were adsorbed to one side of a black lipid membrane, and the Cl- transport activity was measured under different light conditions. Pump activity of halorhodopsin (HR578) induced by green light decreased in time due to the formation of the inactive species HR410L and sodium azide accelerated this inactivation. Additional illumination of the system with blue light restored full activity. The regulatory function of blue light could also be demonstrated in suspensions of cell envelope vesicles and whole cells by the use of passive proton flow as a measure of the chloride transport activity. The antagonistic action of yellow and blue light suggests that halorhodopsin acts as a photochromic pigment in the intact cell