The Transport Activity of the Light Driven Ion Pump Halorhodopsin is regulated 
by Green and Blue Light

Hegemann, Peter; Oesterhelt, Dieter; Bamberg, Ernst

doi:10.1016/0005-2736(85)90174-9

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

The Transport Activity of the Light Driven Ion Pump Halorhodopsin is regulated by Green and Blue Light

MPS-Authors

Hegemann, Peter
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78468

Oesterhelt, Dieter
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons137592

Bamberg, Ernst
Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Hegemann, P., Oesterhelt, D., & Bamberg, E. (1985). The Transport Activity of the Light Driven Ion Pump Halorhodopsin is regulated by Green and Blue Light. Biochimica et Biophysica Acta-Biomembranes, 819(2), 195-205. doi:10.1016/0005-2736(85)90174-9.

Cite as: https://hdl.handle.net/21.11116/0000-0007-A683-9

Abstract

Halorhodopsin-containing membrane fragments or purified halorhodopsin-containing asolectin vesicles were adsorbed to one side of a black lipid membrane, and the Cl^- transport activity was measured under different light conditions. Pump activity of halorhodopsin (HR₅₇₈) induced by green light decreased in time due to the formation of the inactive species HR₄₁₀^L and sodium azide accelerated this inactivation. Additional illumination of the system with blue light restored full activity. The regulatory function of blue light could also be demonstrated in suspensions of cell envelope vesicles and whole cells by the use of passive proton flow as a measure of the chloride transport activity. The antagonistic action of yellow and blue light suggests that halorhodopsin acts as a photochromic pigment in the intact cell