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Chlorophyll b is involved in long-wavelength spectral properties of light-harvesting complexes LHC I and LHC II

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Kühlbrandt,  Werner
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons252478

Rogl,  Hans
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Schmid, V. H., Thomé, P., Rühle, W., Paulsen, H., Kühlbrandt, W., & Rogl, H. (2001). Chlorophyll b is involved in long-wavelength spectral properties of light-harvesting complexes LHC I and LHC II. FEBS Letters, 499(1-2), 27-31. doi:10.1016/S0014-5793(01)02509-1.


Cite as: http://hdl.handle.net/21.11116/0000-0007-6E68-A
Abstract
Chlorophyll (Chl) molecules attached to plant light-harvesting complexes (LHC) differ in their spectral behavior. While most Chl a and Chl b molecules give rise to absorption bands between 645 nm and 670 nm, some special Chls absorb at wavelengths longer than 700 nm. Among the Chl a/b-antennae of higher plants these are found exclusively in LHC I. In order to assign this special spectral property to one chlorophyll species we reconstituted LHC of both photosystem I (Lhca4) and photosystem II (Lhcb1) with carotenoids and only Chl a or Chl b and analyzed the effect on pigment binding, absorption and fluorescence properties. In both LHCs the Chl-binding sites of the omitted Chl species were occupied by the other species resulting in a constant total number of Chls in these complexes. 77-K spectroscopic measurements demonstrated that omission of Chl b in refolded Lhca4 resulted in a loss of long-wavelength absorption and 730-nm fluorescence emission. In Lhcb1 with only Chl b long-wavelength emission was preserved. These results clearly demonstrate the involvement of Chl b in establishing long-wavelength properties.