English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

NeoR, a near-infrared absorbing rhodopsin

MPS-Authors
/persons/resource/persons182767

Seifert,  Reinhard
Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society;

External Resource
Fulltext (public)

s41467-020-19375-8.pdf
(Publisher version), 3MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Broser, M., Spreen, A., Konold, P. E., Peter, E., Adam, S., Borin, V., et al. (2020). NeoR, a near-infrared absorbing rhodopsin. Nature Communications, 11: 5682. doi:10.1038/s41467-020-19375-8.


Cite as: http://hdl.handle.net/21.11116/0000-0007-66CD-0
Abstract
The "Rhizoclosmatium globosum" genome encodes three rhodopsin-guanylyl cyclases (RGCs), which are predicted to facilitate visual orientation of the fungal zoospores. Here, we show that RGC1 and RGC2 function as light-activated cyclases only upon heterodimerization with RGC3 (NeoR). RGC1/2 utilize conventional green or blue-light-sensitive rhodopsins (λmax = 550 and 480 nm, respectively), with short-lived signaling states, responsible for light-activation of the enzyme. The bistable NeoR is photoswitchable between a near-infrared-sensitive (NIR, λmax = 690 nm) highly fluorescent state (QF = 0.2) and a UV-sensitive non-fluorescent state, thereby modulating the activity by NIR pre-illumination. No other rhodopsin has been reported so far to be functional as a heterooligomer, or as having such a long wavelength absorption or high fluorescence yield. Site-specific mutagenesis and hybrid quantum mechanics/molecular mechanics simulations support the idea that the unusual photochemical properties result from the rigidity of the retinal chromophore and a unique counterion triad composed of two glutamic and one aspartic acids. These findings substantially expand our understanding of the natural potential and limitations of spectral tuning in rhodopsin photoreceptors.