English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells

MPS-Authors
/persons/resource/persons204285

Lämmermann,  Tim
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Fulltext (public)

Quast et al..pdf
(Publisher version), 2MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Quast, T., Tappertzhofen, B., Schild, C., Grell, J., Czeloth, N., Förster, R., et al. (2009). Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. Blood, 113, 5801-5810. doi:10.1182/blood-2008-08-176123.


Cite as: http://hdl.handle.net/21.11116/0000-0007-73D8-4
Abstract
Adhesion and motility of mammalian leukocytes are essential requirements for innate and adaptive immune defense mechanisms. We show here that the guanine nucleotide exchange factor cytohesin-1, which had previously been demonstrated to be an important component of beta-2 integrin activation in lymphocytes, regulates the activation of the small GTPase RhoA in primary dendritic cells (DCs). Cytohesin-1 and RhoA are both required for the induction of chemokine-dependent conformational changes of the integrin beta-2 subunit of DCs during adhesion under physiological flow conditions. Furthermore, use of RNAi in murine bone marrow DCs (BM-DCs) revealed that interference with cytohesin-1 signaling impairs migration of wild-type dendritic cells in complex 3D environments and in vivo. This phenotype was not observed in the complete absence of integrins. We thus demonstrate an essential role of cytohesin-1/RhoA during ameboid migration in the presence of integrins and further suggest that DCs without integrins switch to a different migration mode.