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Book Chapter

Using Metabolomics to Assist Plant Breeding

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Alseekh,  S.
The Genetics of Crop Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Fernie,  A. R.
Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Alseekh, S., & Fernie, A. R. (2021). Using Metabolomics to Assist Plant Breeding. In P. Tripodi (Ed.), Crop Breeding: Genetic Improvement Methods (pp. 33-46). New York, NY: Springer US.


Cite as: https://hdl.handle.net/21.11116/0000-0007-85C3-6
Abstract
Recent methodological advances in both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) have provided a deep understanding of metabolic regulation occurring in plant cells. The application of these techniques to agricultural systems is, however, subject to more complex interactions. Here we summarize a step-by-step modern metabolomics methodology that generates metabolome data toward the implementation of metabolomics in crop breeding. We describe a metabolic workflow, and provide guidelines for handling large sample numbers for the specific purpose of metabolic quantitative trait loci approaches.