English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers

MPS-Authors
/persons/resource/persons213391

Wan,  William
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)

elife-59225-v3.pdf
(Publisher version), 7MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Wan, W., Clarke, M., Norris, M. J., Kolesnikova, L., Koehler, A., Bornholdt, Z. A., et al. (2020). Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers. eLife, 9: e59225. doi:10.7554/eLife.59225.


Cite as: http://hdl.handle.net/21.11116/0000-0007-D5A5-E
Abstract
Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.