A conspicuous nickel protein in microbial mats that oxidize methane 
anaerobically

Krüger, M.; Meyerdierks, A.; Glockner, F. O.; Amann, R.; Widdel, F.; Kube, M.; Reinhardt, R.; Kahnt, R.; Böcher, R.; Thauer, R. K.; Shima, S.

doi:10.1038/nature02207

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A conspicuous nickel protein in microbial mats that oxidize methane anaerobically

MPS-Authors

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Krüger, M.
Max Planck Society;

/persons/resource/persons254538

Meyerdierks, A.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

Glockner, F. O.
Max Planck Society;

Amann, R.
Max Planck Society;

Widdel, F.
Max Planck Society;

/persons/resource/persons254470

Kube, M.
Max Planck Society;

Reinhardt, R.
Max Planck Society;

/persons/resource/persons254414

Kahnt, R.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254152

Böcher, R.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254760

Thauer, R. K.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254714

Shima, S.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Department-Independent Research Group Microbial Protein Structure, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1038/nature02207
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Citation

Krüger, M., Meyerdierks, A., Glockner, F. O., Amann, R., Widdel, F., Kube, M., et al. (2003). A conspicuous nickel protein in microbial mats that oxidize methane anaerobically. Nature, 426(6968), 878-881. doi:10.1038/nature02207.

Cite as: https://hdl.handle.net/21.11116/0000-0007-C99C-7

Abstract

Anaerobic oxidation of methane (AOM) in marine sediments is an important microbial process in the global carbon cycle and in control of greenhouse gas emission. The responsible organisms supposedly reverse the reactions of methanogenesis1,2,3,4,5,6,7,8, but cultures providing biochemical proof of this have not been isolated. Here we searched for AOM-associated cell components in microbial mats from anoxic methane seeps in the Black Sea9,10,11. These mats catalyse AOM rather than carry out methanogenesis. We extracted a prominent nickel compound displaying the same absorption spectrum as the nickel cofactor F430 of methyl-coenzyme M reductase, the terminal enzyme of methanogenesis12; however, the nickel compound exhibited a higher molecular mass than F430. The apparent variant of F430 was part of an abundant protein that was purified from the mat and that consists of three different subunits. Determined amino-terminal amino acid sequences matched a gene locus cloned from the mat. Sequence analyses revealed similarities to methyl-coenzyme M reductase from methanogenic archaea. The abundance of the nickel protein (7% of extracted proteins) in the mat suggests an important role in AOM.