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Journal Article

PKA and MAPK phosphorylation of Prf1 allows promoter discrimination in Ustilago maydis.

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Kaffarnik,  Florian
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Mueller,  Philip
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Kahmann,  Regine
Emeriti Molecular Phytopathology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Feldbruegge,  Michael
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Kaffarnik, F., Mueller, P., Leibundgut, M., Kahmann, R., & Feldbruegge, M. (2003). PKA and MAPK phosphorylation of Prf1 allows promoter discrimination in Ustilago maydis. The EMBO Journal, 22(21), 5817-5826. doi:10.1093/emboj/cdg554.


Cite as: https://hdl.handle.net/21.11116/0000-0007-C9BE-1
Abstract
Mating in Ustilago maydis requires cross-talk between cAMP and mitogen-activated protein kinase (MAPK) signalling. During this process, pheromone response factor 1 (Prf1) activates transcription of a and b mating type genes by binding to pheromone response elements (PREs) located in regulatory regions of these genes. Here, we show that PREs are also necessary and sufficient to mediate cAMP-induced gene expression. Prf1 interacts with cAMP-dependent protein kinase A (PKA) Adr1 as well as MAPK Kpp2 in vivo, and its central phosphorylation sites that are functionally important are modified by the respective kinases in vitro. PKA sites in Prf1 are essential for induced expression of a and b mating type genes. In contrast, MAPK sites are not required for pheromone-induced expression of a genes but are crucial for pheromone-responsive b gene expression. This illustrates how a single transcription factor can integrate signals from two pathways and how its phosphorylation status can determine different transcriptional responses.