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Zeitschriftenartikel

Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

MPG-Autoren

Schladt,  T Moritz
Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society;

Berger,  Thomas K
Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society;

Externe Ressourcen

https://www.nature.com/articles/s41598-020-77986-z.pdf
(Verlagsversion)

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Zitation

Schladt, T. M., & Berger, T. K. (2020). Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel. Scientific Reports, 10: 21293. doi:10.1038/s41598-020-77986-z.


Zitierlink: https://hdl.handle.net/21.11116/0000-0007-9759-B
Zusammenfassung
The voltage-gated proton channel Hv1 is expressed in a variety of cells, including macrophages,
sperm, and lung epithelial cells. Hv1 is gated by both the membrane potential and the diference
between the intra- and extracellular pH (ΔpH). The coupling of voltage- and ∆pH-sensing is such
that Hv1 opens only when the electrochemical proton gradient is outwardly directed. However, the
molecular mechanism of this coupling is not known. Here, we investigate the coupling between
voltage- and ΔpH-sensing of Ciona intestinalis proton channel (ciHv1) using patch-clamp fuorometry
(PCF) and proton uncaging. We show that changes in ΔpH can induce conformational changes of the
S4 voltage sensor. Our results are consistent with the idea that S4 can detect both voltage and ΔpH.