English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

MPS-Authors
/persons/resource/persons260209

Schladt,  T. Moritz
Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society;

/persons/resource/persons188561

Berger,  Thomas Klaus
Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society;

External Resource
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Schladt, T. M., & Berger, T. K. (2020). Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel. Scientific Reports, 10: 21293. doi:10.1038/s41598-020-77986-z.


Cite as: http://hdl.handle.net/21.11116/0000-0007-9759-B
Abstract
The voltage-gated proton channel Hv1 is expressed in a variety of cells, including macrophages, sperm, and lung epithelial cells. Hv1 is gated by both the membrane potential and the diference between the intra- and extracellular pH (ΔpH). The coupling of voltage- and ∆pH-sensing is such that Hv1 opens only when the electrochemical proton gradient is outwardly directed. However, the molecular mechanism of this coupling is not known. Here, we investigate the coupling between voltage- and ΔpH-sensing of Ciona intestinalis proton channel (ciHv1) using patch-clamp fuorometry (PCF) and proton uncaging. We show that changes in ΔpH can induce conformational changes of the S4 voltage sensor. Our results are consistent with the idea that S4 can detect both voltage and ΔpH.