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Journal Article

Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

MPS-Authors
/persons/resource/persons228497

Ayyer,  K.
Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free Electron Laser Science;

External Ressource
Fulltext (public)

s41597-020-00745-2.pdf
(Publisher version), 5MB

Supplementary Material (public)

13070546.zip
(Supplementary material), 3KB

Citation

Li, H., Nazari, R., Abbey, B., Alvarez, R., Aquila, A., Ayyer, K., et al. (2020). Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source. Scientific Data, 7: 404. doi:10.1038/s41597-020-00745-2.


Cite as: http://hdl.handle.net/21.11116/0000-0007-979B-0
Abstract
Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.