Molecular architecture of Manduca sexta midgut V1 ATPase visualized by electron 
microscopy

Radermacher, Michael; Ruiz, Teresa; Harvey, William R.; Wieczorek, Helmut; Grüber, Gerhard

doi:10.1016/S0014-5793(99)00739-5

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Molecular architecture of Manduca sexta midgut V₁ ATPase visualized by electron microscopy

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Radermacher, Michael
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Ruiz, Teresa
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Radermacher, M., Ruiz, T., Harvey, W. R., Wieczorek, H., & Grüber, G. (1999). Molecular architecture of Manduca sexta midgut V₁ ATPase visualized by electron microscopy. FEBS Letters, 453(3), 383-386. doi:10.1016/S0014-5793(99)00739-5.

Cite as: https://hdl.handle.net/21.11116/0000-0007-A553-1

Abstract

The structure of the V₁ ATPase from the tobacco hornworm Manduca sexta has been determined from electron micrographs of isolated, negatively stained specimens. The resulting images clearly show a pseudohexagonal arrangement of six equal-sized protein densities, presumably representing the three copies each of subunits A and B, which comprise the headpiece of the enzyme. A seventh density could be observed either centrally or asymmetrically to the hexamer. The maximum diameter of the V₁ complex in the hexagonal projection is 13 nm with each of the six peripheral densities being 3–4 nm in diameter