Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in 
Breast Cancer Cells via Amphiregulin

Bischoff, Philip; Kornhuber, Marja; Dunst, Sebastian; Zell, Jakob; Fauler, Beatrix; Mielke, Thorsten; Taubenberger V, Anna; Guck, Jochen; Oelgeschlaeger, Michael; Schoenfelder, Gilbert

doi:10.1016/j.isci.2020.101683

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_3276456_3

DetailsSummary

Released

Journal Article

Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in Breast Cancer Cells via Amphiregulin

MPS-Authors

/persons/resource/persons241284

Guck, Jochen
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Friedrich-Alexander Universität Erlangen-Nürnberg;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

PIIS2589004220308750.pdf
(Publisher version), 8MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Bischoff, P., Kornhuber, M., Dunst, S., Zell, J., Fauler, B., Mielke, T., et al. (2020). Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in Breast Cancer Cells via Amphiregulin. iScience, 23(11): 101683. doi:10.1016/j.isci.2020.101683.

Cite as: https://hdl.handle.net/21.11116/0000-0007-A892-6

Abstract

Estrogens play an important role in the development and progression of human cancers, particularly in breast cancer. Breast cancer progression depends on the malignant destabilization of adherens junctions (AJs) and disruption of tissue integrity. We found that estrogen receptor alpha (ER alpha) inhibition led to a striking spatial reorganization of AJs and microclustering of E-Cadherin (E-Cad) in the cell membrane of breast cancer cells. This resulted in increased stability of AJs and cell stiffness and a reduction of cell motility. These effects were actomyosindependent and reversible by estrogens. Detailed investigations showed that the ERa target gene and epidermal growth factor receptor (EGFR) ligand Amphiregulin (AREG) essentially regulates AJ reorganization and E-Cad microclustering. Our results not only describe a biological mechanism for the organization of AJs and the modulation of mechanical properties of cells but also provide a new perspective on how estrogens and anti-estrogens might influence the formation of breast tumors.