Methods of preparing well-orientated sols of f-actin containing filaments 
suitable for X-ray diffraction

Popp, D.; Lednev, V. V.; Jahn, W.

doi:10.1016/0022-2836(87)90474-8

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Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction

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Popp, D.
Max Planck Institute for Medical Research, Max Planck Society;

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Jahn, W.
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/0022283687904748/pdf?md5=831425700d435daa88a6d04edfb6c36b&pid=1-s2.0-0022283687904748-main.pdf
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https://doi.org/10.1016/0022-2836(87)90474-8
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Citation

Popp, D., Lednev, V. V., & Jahn, W. (1987). Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction. Journal of Molecular Biology (London), 197(4), 679-684. doi:10.1016/0022-2836(87)90474-8.

Cite as: http://hdl.handle.net/21.11116/0000-0007-A702-A

Abstract

In this paper we describe methods of preparing orientated f-actin and reconstituting thin filaments that are suitable for X-ray diffraction that allow us to analyse the structure of f-actin to at least 15 A resolution (1 A = 0.1 nm). We described problems that occur during the process of orientation and ways of solving them.