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Journal Article

Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction

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Popp,  D.
Max Planck Institute for Medical Research, Max Planck Society;

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Jahn,  W.
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Popp, D., Lednev, V. V., & Jahn, W. (1987). Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction. Journal of Molecular Biology (London), 197(4), 679-684. doi:10.1016/0022-2836(87)90474-8.


Cite as: http://hdl.handle.net/21.11116/0000-0007-A702-A
Abstract
In this paper we describe methods of preparing orientated f-actin and reconstituting thin filaments that are suitable for X-ray diffraction that allow us to analyse the structure of f-actin to at least 15 A resolution (1 A = 0.1 nm). We described problems that occur during the process of orientation and ways of solving them.