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Journal Article

Photocleavable Biotinylated Ligands for Affinity Chromatography

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Thiele,  Christoph
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Fahrenholz,  Falk
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Thiele, C., & Fahrenholz, F. (1994). Photocleavable Biotinylated Ligands for Affinity Chromatography. Analytical Biochemistry, 218(2), 330-337. doi:10.1006/abio.1994.1187.


Cite as: http://hdl.handle.net/21.11116/0000-0007-AA3B-8
Abstract
Two derivatives of the peptide hormone cholecystokinin (CCK-8s) containing a photocleavable o-nitrobenzylester group have been synthesized. One analog, [<sup>3</sup>H]4-(biotin-ϵ-Ahx-oxymethyl)-3-nitrobenzoyl-Gly-Orn(propionyl)-ϵ-Ahx-CCK-8s ([<sup>3</supH]BANA-CCK-8s), was biotinylated, while the other, 4-alanyloxymethyl-3-nitrobenzoyl-ϵ-Ahx-CCK-8s (ANA-CCK-8s) had a free amino group for coupling to amino-reactive affinity matrices. The analogs retained high affinity to both CCK receptors and anti-CCK antibodies. [<sup>3</supH]BANA-CCK-8s was bound to a streptavidin-agarose affinity matrix and was subsequently released by irradiation with uv light of wavelengths >320 nm. The ability to recover a biotinylated peptide ligand from a streptavidin column under very mild conditions should be useful in the purification of specifically binding proteins. As a demonstration of this approach, [<sup>3</supH]BANA-CCK-8s was incubated with anti-CCK-antiserum and was subsequently passed over a streptavidin-agarose affinity matrix. After washing, the bound antibodies were eluted by photocleavage of the affinity ligand. Forty-six percent of the bound antibodies were recovered after 40 min of irradiation. The eluted antibodies showed essentially unchanged binding characteristics. The suitability of ANA-CCK-8s for the purification of anti-CCK antibodies was also demonstrated. The approach may prove to be generally useful in the isolation of labile proteins in an intact form.