Voltage-dependent pump currents of the sarcoplasmic reticulum Ca2+-ATPase in 
planar lipid membranes

Eisenrauch, Andreas; Bamberg, Ernst

doi:10.1016/0014-5793(90)80996-V

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Voltage-dependent pump currents of the sarcoplasmic reticulum Ca²⁺-ATPase in planar lipid membranes

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Eisenrauch, Andreas
Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society;

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Bamberg, Ernst
Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Eisenrauch, A., & Bamberg, E. (1990). Voltage-dependent pump currents of the sarcoplasmic reticulum Ca²⁺-ATPase in planar lipid membranes. FEBS Letters, 268(1), 152-156. doi:10.1016/0014-5793(90)80996-V.

Cite as: https://hdl.handle.net/21.11116/0000-0007-A7BC-9

Abstract

Sarcoplasmic reticulum vesicles containing largely Ca²⁺-ATPase were incorporated into planar lipid membranes. The ATPase was activated by a UV flash-induced concentration jump of ATP from a photolabile caged ATP. Under these conditions stationary pump currents were observed. The dependence of these pump currents on applied voltages was investigated. The current-voltage curve of the Ca²⁺-ATPase shows monotonously increasing pump currents with increasing positive potentials of the ATP containing compartment. This indicates the existence of electrogenic steps in the direction of the transported Ca²⁺ ions. From the extrapolated reversal potentials of the curve is concluded that less than four positive net charges are transported per hydrolyzed ATP.