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Journal Article

Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

MPS-Authors
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Libicher,  Kai
Mutschler, Hannes / Biomimetic Systems, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229974

Mutschler,  Hannes
Mutschler, Hannes / Biomimetic Systems, Max Planck Institute of Biochemistry, Max Planck Society;

External Ressource

http://xlink.rsc.org/?DOI=d0cc06515c
(Supplementary material)

Fulltext (public)

d0cc06515c.pdf
(Publisher version), 3MB

Supplementary Material (public)

d0cc06515c1.pdf
(Supplementary material), 651KB

Citation

Libicher, K., & Mutschler, H. (2020). Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer. Chemical Communications, 56(98), 15426-15429. doi:10.1039/d0cc06515c.


Cite as: http://hdl.handle.net/21.11116/0000-0007-D40E-B
Abstract
The bottom-up construction of bio-inspired systems capable of self-maintenance and reproduction is a central goal in systems chemistry and synthetic biology. A particular challenge in such systems is the continuous regeneration of key proteins required for macromolecular synthesis. Here, we probe self-maintenance of a reconstituted in vitro translation system challenged by serial transfer of selected key proteins. We find that the system can simultaneously regenerate multiple essential polypeptides, which then contribute to the maintenance of protein expression after serial transfer. The presented strategy offers a robust methodology for probing and optimizing continuous self-regeneration of proteins in cell-free environments.