English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Prebiotically Plausible RNA Activation Compatible with Ribozyme-Catalyzed Ligation

MPS-Authors
/persons/resource/persons255725

Song,  Emilie Yeonwha
Mutschler, Hannes / Biomimetic Systems, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons230046

Le Vay,  Kristian
Mutschler, Hannes / Biomimetic Systems, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229974

Mutschler,  Hannes
Mutschler, Hannes / Biomimetic Systems, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

anie.202010918.pdf
(Publisher version), 2MB

Supplementary Material (public)

anie202010918-sup-0001-misc_information.pdf
(Supplementary material), 63MB

Citation

Song, E. Y., Jimenez, E. I., Lin, H., Le Vay, K., Krishnamurthy, R., & Mutschler, H. (2021). Prebiotically Plausible RNA Activation Compatible with Ribozyme-Catalyzed Ligation. Angewandte Chemie International Edition, 60, 2952-2957. doi:10.1002/anie.202010918.


Cite as: https://hdl.handle.net/21.11116/0000-0007-AC6C-F
Abstract
RNA-catalyzed RNA ligation is widely believed to be a key reaction for primordial biology. However, since typical chemical routes towards activating RNA substrates are incompatible with ribozyme catalysis, it remains unclear how prebiotic systems generated and sustained pools of activated building blocks needed to form increasingly larger and complex RNA. Herein, we demonstrate in situ activation of RNA substrates under reaction conditions amenable to catalysis by the hairpin ribozyme. We found that diamidophosphate (DAP) and imidazole drive the formation of 2 ',3 '-cyclic phosphate RNA mono- and oligonucleotides from monophosphorylated precursors in frozen water-ice. This long-lived activation enables iterative enzymatic assembly of long RNAs. Our results provide a plausible scenario for the generation of higher-energy substrates required to fuel ribozyme-catalyzed RNA synthesis in the absence of a highly evolved metabolism.