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Journal Article

Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

MPS-Authors
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Auer,  Alexander
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Woehrstein,  Johannes B.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Schueder,  Florian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Strauss,  Maximilian T.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Schnitzbauer,  Joerg
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Jungmann,  Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

Fulltext (public)

s41467-020-18910-x.pdf
(Publisher version), 3MB

Supplementary Material (public)

41467_2020_18910_MOESM16_ESM.pdf
(Supplementary material), 28MB

Citation

Wickham, S. F. J., Auer, A., Min, J., Ponnuswamy, N., Woehrstein, J. B., Schueder, F., et al. (2020). Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard. Nature Communications, 11(1): 5768. doi:10.1038/s41467-020-18910-x.


Cite as: http://hdl.handle.net/21.11116/0000-0007-AC65-6
Abstract
DNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of similar to 90nm diameter and similar to 250nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of similar to 8nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.