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Journal Article

Inhibition of the rat renal Na+/H+ exchanger by beta-adrenergic antagonists


Burckhardt,  Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Friedrich, T., & Burckhardt, G. (1991). Inhibition of the rat renal Na+/H+ exchanger by beta-adrenergic antagonists. Biochemical and Biophysical Research Communications, 175(1), 311-317. doi:10.1016/s0006-291x(05)81236-7.

Cite as: http://hdl.handle.net/21.11116/0000-0007-B120-C
The beta-adrenergic antagonists, alprenolol and propranolol, inhibit the N+/H+ exchanger in rat renal brush-border membrane vesicles. Half-maximal inhibition occurs at 86 microM alprenolol and 36 microM propranolol. Similar to amiloride and Na+, propranolol protects the N+/H+ exchanger from irreversible inhibition by the carboxyl group reagent, N,N'-dicyclohexyl-carbodiimide (DCCD). Protection is incomplete, depends on propranolol concentration, and reaches a maximum at 0.4 mM propranolol. With a comparable dose dependence, propranolol protects a 65 kDa band from labeling with [14C]DCCD. The data indicate that beta-adrenergic antagonists specifically interact with the proximal tubular Na+/H+ exchanger.