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Inhibition of sodium-dependent transport systems in rat renal brush-border membranes with N,N'-dicyclohexylcarbodiimide

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Friedrich,  Thomas
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Sablotni,  Jutta
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Burckhardt,  Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Friedrich, T., Sablotni, J., & Burckhardt, G. (1987). Inhibition of sodium-dependent transport systems in rat renal brush-border membranes with N,N'-dicyclohexylcarbodiimide. Biochemical and Biophysical Research Communications, 147(1), 375-381. doi:10.1016/s0006-291x(87)80132-8.


Cite as: https://hdl.handle.net/21.11116/0000-0007-B2A9-1
Abstract
Treatment of rat renal brush-border membrane vesicles with N,N'-dicyclohexylcarbodiimide (DCCD) causes irreversible inhibition of the Na+-coupled transport systems for D-glucose, L-phenylalanine, L-glutamate, and sulfate. The DCCD-reactive side groups of these transport systems differ in their sensitivity towards DCCD and protection by substrates. The D-glucose and L-glutamate transporters cannot be protected by their substrates. In contrast, Na+ protects the transport systems for L-phenylalanine and sulfate from inactivation by DCCD. The data suggest covalent modification by DCCD of D-glucose and L-glutamate transporters apart from their substrate binding sites and of L-phenylalanine and sulfate transporters within their Na+-binding regions.