Photoaffinity labeling studies of the rat renal sodium bile salt cotransport 
system

Burckhardt, Gerhard; Kramer, Werner; Kurtz, Gerhart; Wilson, Frederick A.

doi:10.1016/0006-291x(87)90353-6

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Photoaffinity labeling studies of the rat renal sodium bile salt cotransport system

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Burckhardt, Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Burckhardt, G., Kramer, W., Kurtz, G., & Wilson, F. A. (1987). Photoaffinity labeling studies of the rat renal sodium bile salt cotransport system. Biochemical and Biophysical Research Communications, 143(3), 1018-1023. doi:10.1016/0006-291x(87)90353-6.

Cite as: https://hdl.handle.net/21.11116/0000-0007-B2A6-4

Abstract

The uptake of a photolabile taurocholate derivative, (7,7-azo-3 alpha, 12 alpha-dihydroxy-5 beta-cholan-24-oyl)-2-aminoethanesulfonate, 7,7-azo-TC, into rat renal brush-border membrane vesicles was stimulated by Na⁺ and inhibited by taurocholate indicating an interaction with the Na⁺/bile salt cotransport system. Irradiation of membrane vesicles in the presence of 7,7-azo-TC inhibited Na⁺-dependent taurocholate uptake irreversibly. Photoaffinity labeling with [³H]7,7-azo-TC resulted in a predominant incorporation of radioactivity into a polypeptide with apparent molecular weight of 99,000. These results suggest that the proteins involved in Na⁺/bile salt cotransport are similar in renal and ileal brush-border membranes, but differ from those in hepatocytes.