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Contraluminal para-aminohippurate (PAH) transport in the proximal tubule of the rat kidney. I. Kinetics, influence of cations, anions, and capillary preperfusion

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Ullrich,  Karl Julius
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Rumrich,  Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Fritzsch,  Günter
Department of Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Klöss,  Sonja
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Ullrich, K. J., Rumrich, G., Fritzsch, G., & Klöss, S. (1987). Contraluminal para-aminohippurate (PAH) transport in the proximal tubule of the rat kidney. I. Kinetics, influence of cations, anions, and capillary preperfusion. Pflügers Archiv: European Journal of Physiology, 409(3), 229-235. doi:10.1007/bf00583470.


Cite as: http://hdl.handle.net/21.11116/0000-0007-DC56-1
Abstract
In order to study the characteristics of contraluminal para-aminohippurate transport into proximal tubular cells the stopped flow capillary perfusion method was applied. The disappearance of 3H-para-aminohippurate from the capillary perfusate at different concentrations and contact times was measured and saturation type behaviour was found with a Km of 0.08 +/- 0.01 (SE) mmol/l, Jmax of 1.1 +/- 0.1 pmol X s-1 X cm-1 and r, the final extracellular/intracellular distribution ratio of 0.93 +/- 0.03. Omission of Na+ from the capillary test perfusate caused a small reduction of contraluminal PAH uptake at small transport rates (0.1 mmol/l PAH in the test perfusate) but not at high transport rates (1.0 mmol/l PAH in the test perfusate). Change of K+ between 0 and 40 mmol/l and pH between 6.0 and 8.0 did not influence contraluminal PAH uptake. Isotonic replacement of chloride by gluconate, nitrate, sulfate, phosphate, methanesulfonate or increase in bicarbonate to 50 mmol/l did not influence PAH uptake at small transport rates. But isotonic sulfate and phosphate, as well as 50 mmol/l HCO3- and 25 mmol/l Hepes in isotonic solutions reduced PAH uptake at high transport rates. Addition of 5 mmol/l Ca2+, Mg2+, Mn2+, Ba2+, Cd2+ to isotonic Na+-gluconate solution did not influence PAH uptake except for Mg2+ and Mn2+ which inhibited uptake at small transport rates only. Preperfusion of the peritubular capillaries with rat serum, Na+ gluconate (Ca2+- + Mg2+-free), Na+ gluconate (Ca2+- + Mg2+-free) plus 10 mmol/l lactate or pyruvate or 0.1 mmol/l 2-oxoglutarate did not influence PAH uptake at small PAH transport rates, but inhibited at high transport rates.