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Journal Article

Far-red switching DNA probes for live cell nanoscopy

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Bucevicius,  J.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Gilat,  T.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Citation

Bucevicius, J., Gilat, T., & Lukinavicius, G. (2020). Far-red switching DNA probes for live cell nanoscopy. Chemical Communications, 56(94), 14797-14800. doi:10.1039/D0CC06759H.


Cite as: https://hdl.handle.net/21.11116/0000-0007-D7F0-7
Abstract

Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5′-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.