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Comparison of target enrichment strategies for ancient pathogen DNA

MPS-Authors

Furtwängler,  Anja
MHAAM, Max Planck Institute for the Science of Human History, Max Planck Society;

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Krause-Kyora,  Ben
Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society;

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Krause,  Johannes
Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society;
MHAAM, Max Planck Institute for the Science of Human History, Max Planck Society;

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Herbig,  Alexander
Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society;

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Citation

Furtwängler, A., Neukamm, J., Böhme, L., Reiter, E., Vollstedt, M., Arora, N., et al. (2020). Comparison of target enrichment strategies for ancient pathogen DNA. Biotechniques, 69(6), 455-459. doi:10.2144/btn-2020-0100.


Cite as: http://hdl.handle.net/21.11116/0000-0007-DB89-8
Abstract
In ancient DNA research, the degraded nature of the samples generally results in poor yields of highly fragmented DNA; targeted DNA enrichment is thus required to maximize research outcomes. The three commonly used methods ? array-based hybridization capture and in-solution capture using either RNA or DNA baits ? have different characteristics that may influence the capture efficiency, specificity and reproducibility. Here we compare their performance in enriching pathogen DNA of Mycobacterium leprae and Treponema pallidum from 11 ancient and 19 modern samples. We find that in-solution approaches are the most effective method in ancient and modern samples of both pathogens and that RNA baits usually perform better than DNA baits.